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anti abcg2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti abcg2
    Anti Abcg2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+abcg2/ABCG2+XP+Rabbit+mAb/pm41898529-203-15-16
    Average 95 stars, based on 137 article reviews
    anti abcg2 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Ticagrelor reverses multidrug resistance in breast cancer by inhibiting PI3K/AKT/mTOR pathway and suppressing ABCB1 expression and function.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Genome-Wide CRISPR Screens Identify ABCG2-Mediated Drug Resistance to the Threonine Tyrosine Kinase (TTK) Inhibitor CFI-402257 in Breast Cancer.
    Article Snippet: Lentiviruses encoding these constructs, as well as pLenti-Guide-GFP and pLenti-Guide-mCh (Addgene #185474) constructs containing an sgRNA targeting AAVS1 as a negative control, were produced and used to infect Cas9+ cells to generate cells expressing mCh+sgAAVS1, GFP+sgAAVS1, GFP+sgABCG2ex4KD, and GFP+sgABCG2ex6KD. pLenti-Guide vectors were generously provided by Dr. Daniel Durocher. .. ABCG2 KD and OE were confirmed with RT-qPCR using SYBR-green reagent and/or Western blotting using anti-ABCG2 (Cell Signaling #42078 at 1:500 dilution, Danvers, MA, USA) and anti-GAPDH (Cell Signaling #2118 at 1:1000 dilution) antibodies. qPCR primer sequences are provided in Table S1. ..

    Article Title: Genome-Wide CRISPR Screens Identify ABCG2-Mediated Drug Resistance to the Threonine Tyrosine Kinase (TTK) Inhibitor CFI-402257 in Breast Cancer
    Article Snippet: Lentiviruses encoding these constructs, as well as pLenti-Guide-GFP and pLenti-Guide-mCh (Addgene #185474) constructs containing an sgRNA targeting AAVS1 as a negative control, were produced and used to infect Cas9+ cells to generate cells expressing mCh+sgAAVS1, GFP+sgAAVS1, GFP+sgABCG2ex4 KD , and GFP+sgABCG2ex6 KD . pLenti-Guide vectors were generously provided by Dr. Daniel Durocher. .. ABCG2 KD and OE were confirmed with RT-qPCR using SYBR-green reagent and/or Western blotting using anti-ABCG2 (Cell Signaling #42078 at 1:500 dilution, Danvers, MA, USA) and anti-GAPDH (Cell Signaling #2118 at 1:1000 dilution) antibodies. qPCR primer sequences are provided in . ..

    Quantitative RT-PCR:

    Article Title: Genome-Wide CRISPR Screens Identify ABCG2-Mediated Drug Resistance to the Threonine Tyrosine Kinase (TTK) Inhibitor CFI-402257 in Breast Cancer.
    Article Snippet: Lentiviruses encoding these constructs, as well as pLenti-Guide-GFP and pLenti-Guide-mCh (Addgene #185474) constructs containing an sgRNA targeting AAVS1 as a negative control, were produced and used to infect Cas9+ cells to generate cells expressing mCh+sgAAVS1, GFP+sgAAVS1, GFP+sgABCG2ex4KD, and GFP+sgABCG2ex6KD. pLenti-Guide vectors were generously provided by Dr. Daniel Durocher. .. ABCG2 KD and OE were confirmed with RT-qPCR using SYBR-green reagent and/or Western blotting using anti-ABCG2 (Cell Signaling #42078 at 1:500 dilution, Danvers, MA, USA) and anti-GAPDH (Cell Signaling #2118 at 1:1000 dilution) antibodies. qPCR primer sequences are provided in Table S1. ..

    Article Title: Genome-Wide CRISPR Screens Identify ABCG2-Mediated Drug Resistance to the Threonine Tyrosine Kinase (TTK) Inhibitor CFI-402257 in Breast Cancer
    Article Snippet: Lentiviruses encoding these constructs, as well as pLenti-Guide-GFP and pLenti-Guide-mCh (Addgene #185474) constructs containing an sgRNA targeting AAVS1 as a negative control, were produced and used to infect Cas9+ cells to generate cells expressing mCh+sgAAVS1, GFP+sgAAVS1, GFP+sgABCG2ex4 KD , and GFP+sgABCG2ex6 KD . pLenti-Guide vectors were generously provided by Dr. Daniel Durocher. .. ABCG2 KD and OE were confirmed with RT-qPCR using SYBR-green reagent and/or Western blotting using anti-ABCG2 (Cell Signaling #42078 at 1:500 dilution, Danvers, MA, USA) and anti-GAPDH (Cell Signaling #2118 at 1:1000 dilution) antibodies. qPCR primer sequences are provided in . ..

    Real-time Polymerase Chain Reaction:

    Article Title: Genome-Wide CRISPR Screens Identify ABCG2-Mediated Drug Resistance to the Threonine Tyrosine Kinase (TTK) Inhibitor CFI-402257 in Breast Cancer.
    Article Snippet: Lentiviruses encoding these constructs, as well as pLenti-Guide-GFP and pLenti-Guide-mCh (Addgene #185474) constructs containing an sgRNA targeting AAVS1 as a negative control, were produced and used to infect Cas9+ cells to generate cells expressing mCh+sgAAVS1, GFP+sgAAVS1, GFP+sgABCG2ex4KD, and GFP+sgABCG2ex6KD. pLenti-Guide vectors were generously provided by Dr. Daniel Durocher. .. ABCG2 KD and OE were confirmed with RT-qPCR using SYBR-green reagent and/or Western blotting using anti-ABCG2 (Cell Signaling #42078 at 1:500 dilution, Danvers, MA, USA) and anti-GAPDH (Cell Signaling #2118 at 1:1000 dilution) antibodies. qPCR primer sequences are provided in Table S1. ..

    Article Title: Genome-Wide CRISPR Screens Identify ABCG2-Mediated Drug Resistance to the Threonine Tyrosine Kinase (TTK) Inhibitor CFI-402257 in Breast Cancer
    Article Snippet: Lentiviruses encoding these constructs, as well as pLenti-Guide-GFP and pLenti-Guide-mCh (Addgene #185474) constructs containing an sgRNA targeting AAVS1 as a negative control, were produced and used to infect Cas9+ cells to generate cells expressing mCh+sgAAVS1, GFP+sgAAVS1, GFP+sgABCG2ex4 KD , and GFP+sgABCG2ex6 KD . pLenti-Guide vectors were generously provided by Dr. Daniel Durocher. .. ABCG2 KD and OE were confirmed with RT-qPCR using SYBR-green reagent and/or Western blotting using anti-ABCG2 (Cell Signaling #42078 at 1:500 dilution, Danvers, MA, USA) and anti-GAPDH (Cell Signaling #2118 at 1:1000 dilution) antibodies. qPCR primer sequences are provided in . ..

    Blocking Assay:

    Article Title: Multi-Center “Replica Study” Challenges the Impact of Electronic Cigarette Aerosols on Cisplatin Resistance in Head and Neck Cancer Cells
    Article Snippet: .. After blocking the nonspecific sites, the membranes were incubated overnight at room temperature with the following primary antibodies resuspended in 5% BSA solution: anti-ATP7A (1:100; Santa Cruz Biotechnologies, Texas, USA, sc-376467), anti-CTR1 (1:40.000; Abcam, Massachusetts, USA, #ab129067), anti-ABCG2 (1:100; Cell Signaling Technology, Massachusetts, USA, #42078), and β-actin as a loading control (1:20.000; Cell Signaling Technology, Massachusetts, USA, #8457). .. After incubation, the membranes were washed three times with Tris-buffered saline with 0.1% Tween 20 (TBS-T), and subsequently incubated for 1h at room temperature with the following secondary antibodies resuspended in 5% BSA solution: rabbit anti-mouse IgG (1:10.000; Abcam, Massachusetts, USA, #ab6728) and goat anti-Rabbit IgG (1:10.000; Cell Signaling Technology, Massachusetts, USA, #7074).

    Incubation:

    Article Title: Multi-Center “Replica Study” Challenges the Impact of Electronic Cigarette Aerosols on Cisplatin Resistance in Head and Neck Cancer Cells
    Article Snippet: .. After blocking the nonspecific sites, the membranes were incubated overnight at room temperature with the following primary antibodies resuspended in 5% BSA solution: anti-ATP7A (1:100; Santa Cruz Biotechnologies, Texas, USA, sc-376467), anti-CTR1 (1:40.000; Abcam, Massachusetts, USA, #ab129067), anti-ABCG2 (1:100; Cell Signaling Technology, Massachusetts, USA, #42078), and β-actin as a loading control (1:20.000; Cell Signaling Technology, Massachusetts, USA, #8457). .. After incubation, the membranes were washed three times with Tris-buffered saline with 0.1% Tween 20 (TBS-T), and subsequently incubated for 1h at room temperature with the following secondary antibodies resuspended in 5% BSA solution: rabbit anti-mouse IgG (1:10.000; Abcam, Massachusetts, USA, #ab6728) and goat anti-Rabbit IgG (1:10.000; Cell Signaling Technology, Massachusetts, USA, #7074).

    Control:

    Article Title: Multi-Center “Replica Study” Challenges the Impact of Electronic Cigarette Aerosols on Cisplatin Resistance in Head and Neck Cancer Cells
    Article Snippet: .. After blocking the nonspecific sites, the membranes were incubated overnight at room temperature with the following primary antibodies resuspended in 5% BSA solution: anti-ATP7A (1:100; Santa Cruz Biotechnologies, Texas, USA, sc-376467), anti-CTR1 (1:40.000; Abcam, Massachusetts, USA, #ab129067), anti-ABCG2 (1:100; Cell Signaling Technology, Massachusetts, USA, #42078), and β-actin as a loading control (1:20.000; Cell Signaling Technology, Massachusetts, USA, #8457). .. After incubation, the membranes were washed three times with Tris-buffered saline with 0.1% Tween 20 (TBS-T), and subsequently incubated for 1h at room temperature with the following secondary antibodies resuspended in 5% BSA solution: rabbit anti-mouse IgG (1:10.000; Abcam, Massachusetts, USA, #ab6728) and goat anti-Rabbit IgG (1:10.000; Cell Signaling Technology, Massachusetts, USA, #7074).

    other:

    Article Title: ENOX2 (tNOX)–Associated Stemness in Oral Cancer Cells and Its Clinical Correlation in Head and Neck Tumors
    Article Snippet: The following antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA): anti-SIRT1 (1:1000, Cat#2496), anti-Nanog (1:2000, Cat#4903), anti-SOX2 (1:1000, Cat#2748), anti-Oct4 (1:1000, Cat#2750), anti-ABCG2 (1:1000, Cat#4477), anti-ALDH1 (1:1000, Cat#54135), anti-PKCδ (1:1000, Cat#2058), anti-c-Myc (1:1000, Cat#5605), and anti-GST (1:1000, Cat#2624).

    Article Title: Kaempferol attenuates hyperuricemia combined with gouty arthritis via urate transporters and NLRP3/NF-κB pathway modulation
    Article Snippet: Anti-ABCG2 , Cell Signaling Technology , Cat# 42078; RRID: AB_2799211.



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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Image Search Results


    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of ABCG2 in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.

    Journal: Frontiers in Nutrition

    Article Title: Oral L-carnitine supplementation lowers serum uric acid and is associated with renal and intestinal urate-handling responses in hyperuricemic mice

    doi: 10.3389/fnut.2026.1866695

    Figure Lengend Snippet: Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of ABCG2 in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.

    Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: XOD (1:2000, Cat. No. 55156-1-AP, Proteintech), ADA (1:2000, Cat. No. 67870-1-Ig, Proteintech), OAT1 (1:2000, Cat. No. 26574-1-AP, Proteintech), OAT3 (1:2000, Cat. No. bs-0609R, Bioss), GLUT9 (1:1000, Cat. No. 26486-1-AP, Proteintech), ABCG2 (1:1000, Cat. No. 27286-1-AP, Proteintech), URAT1 (1:3000, Cat. No. 14937-1-AP, Proteintech), IκBα (1:1000, Cat. No. GB111509 , Servicebio), NF-κB p65 (1:2000, Cat. No. 66535-1-Ig, Proteintech), phospho-NF-κB p65 (1:2000, Cat. No. ZHS1565, ZhongHe) and β-actin (1:8000, Cat. No. 66009-1-Ig, Proteintech).

    Techniques: Morphology, Expressing, Staining, Tissue, Immunofluorescence, Analysis

    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Journal: STAR Protocols

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays

    doi: 10.1016/j.xpro.2026.104361

    Figure Lengend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Article Snippet: Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) , Santa Cruz Biotechnology , Cat#sc-58222; RRID: AB_630828.

    Techniques: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence